Primary antibody incubation time
During the detection step, the complex of the protein, antibody, and antibody is found on the membrane. Detection systems can be based on chemiluminescence, chemifluorescence, fluorescence, chromogenic, or radioisotopic detection. You can use a film, a CCD camera, or a scanner to collect the light given off by the detection process during the capturing step. Most of the time, it's easy to figure out what the data from a Western blot means.
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